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CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity

The knirps (kni) locus encodes transcription factors required for induction of the L2 wing vein in Drosophila. Here, we employ diverse CRISPR/Cas9 genome editing tools to generate a series of targeted lesions within the endogenous cis-regulatory module (CRM) required for kni expression in the L2 vei...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Xu, Xiang-Ru Shannon, Gantz, Valentino Matteo, Siomava, Natalia, Bier, Ethan
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5800851/
https://ncbi.nlm.nih.gov/pubmed/29274230
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.30281
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