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Generation of a rod-specific NRL reporter line in human pluripotent stem cells

Reporter lines generated in human pluripotent stem cells can be highly useful for the analysis of specific cell types and lineages in live cultures. We created the first human rod reporter line using CRISPR/Cas9 genome editing to replace one allele of the Neural Retina Leucine zipper (NRL) gene with...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Phillips, M. Joseph, Capowski, Elizabeth E., Petersen, Andrew, Jansen, Alex D., Barlow, Katherine, Edwards, Kimberly L., Gamm, David M.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5799252/
https://ncbi.nlm.nih.gov/pubmed/29402929
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-20813-3
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