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Mass Cytometry Assays for Antigen-Specific T Cells Using CyTOF
T Cells specific for a single antigen tend to be rare, even after expansion of memory cells. They are commonly detected by in vitro stimulation with peptides or protein, followed by staining for intracellular cytokines. In this protocol, we use CyTOF(®) mass cytometry to collect single-cell data on...
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| Published in: | Methods Mol Biol |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5798871/ https://ncbi.nlm.nih.gov/pubmed/29071674 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7346-0_3 |
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