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Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA

We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity. Th...

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Detalhes bibliográficos
Publicado no:Braz J Microbiol
Main Authors: Feng, Na, Zhou, Yazhou, Fan, Yanxiao, Bi, Yujing, Yang, Ruifu, Zhou, Yusen, Wang, Xiaoyi
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5790586/
https://ncbi.nlm.nih.gov/pubmed/28887007
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bjm.2017.03.014
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