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Comparing two approaches of miR-34a target identification, biotinylated-miRNA pulldown vs miRNA overexpression

microRNAs (miRNAs) are critical regulators of gene expression. For elucidating functional roles of miRNAs, it is critical to identify their direct targets. There are debates about whether pulldown of biotinylated miRNA mimics can be used to identify miRNA targets or not. Here we show that biotin-lab...

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Detalhes bibliográficos
Publicado no:RNA Biol
Main Authors: Awan, Hassaan Mehboob, Shah, Abdullah, Rashid, Farooq, Wei, Shuai, Chen, Liang, Shan, Ge
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5786020/
https://ncbi.nlm.nih.gov/pubmed/29028450
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2017.1391441
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