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Mixed pyruvate labeling enables backbone resonance assignment of large proteins using a single experiment
Backbone resonance assignment is a critical first step in the investigation of proteins by NMR. This is traditionally achieved with a standard set of experiments, most of which are not optimal for large proteins. Of these, HNCA is the most sensitive experiment that provides sequential correlations....
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| Publicat a: | Nat Commun |
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| Autors principals: | , , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group UK
2018
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5783931/ https://ncbi.nlm.nih.gov/pubmed/29367739 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-02767-8 |
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