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Isolation of Newly Transcribed RNA Using the Metabolic Label 4-thiouridine
Isolation of newly transcribed RNA is an invaluable approach that can be used to study the dynamic life of RNA in cellulo. Traditional methods of whole-cell RNA extraction limit subsequent gene expression analyses to the steady-state levels of RNA abundance, which often masks changes in RNA synthesi...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5783291/ https://ncbi.nlm.nih.gov/pubmed/28766297 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7204-3_13 |
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