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Two Complementary Reversed-Phase Separations Achieve Comprehensive Coverage of the Semipolar and Nonpolar Metabolome

Although it is common in untargeted metabolomics to apply reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) methods that have been systematically optimized for lipids and central carbon metabolites, here we show that these established protocols pro...

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Publicado en:Anal Bioanal Chem
Autores principales: Naser, Fuad J., Mahieu, Nathaniel G., Wang, Lingjue, Spalding, Jonathan L., Johnson, Stephen L., Patti, Gary J.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2017
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5776055/
https://ncbi.nlm.nih.gov/pubmed/29256075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-017-0768-x
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