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Modulating Gene Expression in Epstein-Barr Virus (EBV)-Positive B Cell Lines with CRISPRa and CRISPRi
EBV transforms small resting primary B cells into large lymphoblastoid cells which are able to grow and survive in vitro indefinitely. These cells represent a model for oncogenesis. In this unit, variants of conventional Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR), namely the a...
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| Publicado en: | Curr Protoc Mol Biol |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2018
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5774230/ https://ncbi.nlm.nih.gov/pubmed/29337370 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmb.50 |
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