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A surrogate reporter system for multiplexable evaluation of CRISPR/Cas9 in targeted mutagenesis
Engineered nucleases in genome editing manifest diverse efficiencies at different targeted loci. There is therefore a constant need to evaluate the mutation rates at given loci. T7 endonuclease 1 (T7E1) and Surveyor mismatch cleavage assays are the most widely used methods, but they are labour and t...
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| Опубликовано в: : | Sci Rep |
|---|---|
| Главные авторы: | , , , , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Nature Publishing Group UK
2018
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5773543/ https://ncbi.nlm.nih.gov/pubmed/29348585 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-19317-x |
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