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High-resolution imaging in two-photon excitation microscopy using in situ estimations of the point spread function

We present a technique for improving the spatial resolution of two-photon excitation microscopy; our technique combines annular illumination with an in situ estimation of the point spread function (PSF) used for deconvolution. For the in situ estimation of the PSF, we developed a technique called au...

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Detalhes bibliográficos
Publicado no:Biomed Opt Express
Main Authors: Doi, Atsushi, Oketani, Ryosuke, Nawa, Yasunori, Fujita, Katsumasa
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5772575/
https://ncbi.nlm.nih.gov/pubmed/29359097
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.9.000202
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