In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteria

BACKGROUND: Fluorescent reporter proteins (FP) have become an indispensable tool for the optimization of microbial cell factories and in synthetic biology per se. The applicability of the currently available FPs is, however, constrained by species-dependent performance and misfolding at elevated tem...

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Bibliographic Details
Published in:Biotechnol Biofuels
Main Authors: Frenzel, Elrike, Legebeke, Jelmer, van Stralen, Atze, van Kranenburg, Richard, Kuipers, Oscar P.
Format: Artigo
Language:Inglês
Published: BioMed Central 2018
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Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5771013/
https://ncbi.nlm.nih.gov/pubmed/29371884
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13068-017-1008-5
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