Rapid and Efficient CRISPR/Cas9-Based Mating-Type Switching of Saccharomyces cerevisiae

Rapid and highly efficient mating-type switching of Saccharomyces cerevisiae enables a wide variety of genetic manipulations, such as the construction of strains, for instance, isogenic haploid pairs of both mating-types, diploids and polyploids. We used the CRISPR/Cas9 system to generate a double-s...

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Veröffentlicht in:G3 (Bethesda)
Hauptverfasser: Xie, Ze-Xiong, Mitchell, Leslie A., Liu, Hui-Min, Li, Bing-Zhi, Liu, Duo, Agmon, Neta, Wu, Yi, Li, Xia, Zhou, Xiao, Li, Bo, Xiao, Wen-Hai, Ding, Ming-Zhu, Wang, Ying, Yuan, Ying-Jin, Boeke, Jef D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Genetics Society of America 2017
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Investigations
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5765346/
https://ncbi.nlm.nih.gov/pubmed/29150593
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.117.300347
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