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An optimized method for 3D fluorescence co-localization applied to human kinetochore protein architecture

Two-color fluorescence co-localization in 3D (three-dimension) has the potential to achieve accurate measurements at the nanometer length scale. Here, we optimized a 3D fluorescence co-localization method that uses mean values for chromatic aberration correction to yield the mean separation with ~10...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Suzuki, Aussie, Long, Sarah K, Salmon, Edward D
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5764572/
https://ncbi.nlm.nih.gov/pubmed/29323636
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.32418
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