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Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin–Microtubule Complexes
Single molecule fluorescence polarization microscopy (smFPM) is a technique that enables to monitor changes in the orientation of a single labeled protein domain. Here we describe a smFPM microscope set-up and protocols to investigate conformational changes associated with the movement of motor prot...
Sparad:
| I publikationen: | Methods Mol Biol |
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| Huvudupphovsmän: | , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
2018
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5760212/ https://ncbi.nlm.nih.gov/pubmed/28940071 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7271-5_11 |
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