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Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

The advent of 3D in vitro organoids that mimic the in vivo tissue architecture and morphogenesis has greatly advanced the ability to study key biological questions in cell and developmental biology. In addition, organoids together with recent technical advances in gene editing and viral gene deliver...

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Podrobná bibliografie
Vydáno v:J Vis Exp
Hlavní autoři: Goldspink, Deborah A., Matthews, Zoe J., Lund, Elizabeth K., Wileman, Tom, Mogensen, Mette M.
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5755565/
https://ncbi.nlm.nih.gov/pubmed/29286416
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56662
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