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A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

Post-translational modifications that occur at specific positions of proteins have been shown to play important roles in a variety of cellular processes. Among them, reversible lysine acetylation is one of the most widely distributed in all domains of life. Although numerous mass spectrometry-based...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Venkat, Sumana, Gregory, Caroline, Meng, Kexin, Gan, Qinglei, Fan, Chenguang
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5755542/
https://ncbi.nlm.nih.gov/pubmed/29286490
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57061
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