Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Proteins interact with their ligands to form active and dynamic assemblies which carry out various cellular functions. Elucidating these interactions is therefore fundamental for the understanding of cellular processes. However, many protein complexes are dynamic assemblies and are not accessible by...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Haupt, Caroline, Hofmann, Tommy, Wittig, Sabine, Kostmann, Susann, Politis, Argyris, Schmidt, Carla
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2017
Subjects:
Biochemistry
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5755487/
https://ncbi.nlm.nih.gov/pubmed/29286378
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56747
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