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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Proteins interact with their ligands to form active and dynamic assemblies which carry out various cellular functions. Elucidating these interactions is therefore fundamental for the understanding of cellular processes. However, many protein complexes are dynamic assemblies and are not accessible by...

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Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: Haupt, Caroline, Hofmann, Tommy, Wittig, Sabine, Kostmann, Susann, Politis, Argyris, Schmidt, Carla
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2017
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5755487/
https://ncbi.nlm.nih.gov/pubmed/29286378
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56747
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