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Gaussian decomposition of high-resolution melt curve derivatives for measuring genome-editing efficiency
We describe a method for measuring genome editing efficiency from in silico analysis of high-resolution melt curve data. The melt curve data derived from amplicons of genome-edited or unmodified target sites were processed to remove the background fluorescent signal emanating from free fluorophore a...
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| Publicado no: | PLoS One |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5754072/ https://ncbi.nlm.nih.gov/pubmed/29300734 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0190192 |
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