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Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Elucidation of the binding properties of chromatin-targeting proteins can be very challenging due to the complex nature of chromatin and the heterogeneous nature of most mammalian chromatin-modifying complexes. In order to overcome these hurdles, we have adapted a sequential salt extraction (SSE) as...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Porter, Elizabeth G., Connelly, Katelyn E., Dykhuizen, Emily C.
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5752352/
https://ncbi.nlm.nih.gov/pubmed/28994797
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55369
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