Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

The aim of this protocol presents an optimized procedure for the purification and cultivation of pBECs and to establish in vitro blood-brain barrier (BBB) models based on pBECs in mono-culture (MC), MC with astrocyte-conditioned medium (ACM), and non-contact co-culture (NCC) with astrocytes of porci...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Nielsen, Simone S. E., Siupka, Piotr, Georgian, Ana, Preston, Jane E., Tóth, Andrea E., Yusof, Siti R., Abbott, N. Joan, Nielsen, Morten S.
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2017
Schlagworte:
Medicine
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5752332/
https://ncbi.nlm.nih.gov/pubmed/28994773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56277
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