Validation of Anti-Mouse PDL-1 Goat Polyclonal Antibody Staining with Mouse PDL-1 In Situ Hybridization on Adjacent Sections of Cell Pellets and Mouse Tumors

Finding a valid antibody to detect mouse programmed death ligand 1 (PDL-1) by immunohistochemistry or immunofluorescence staining has been notoriously difficult. Successful validation of an antibody requires the use of multiple detection methods with the ability to compare appropriate positive and n...

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Veröffentlicht in:Methods Mol Biol
Hauptverfasser: Ricca, Jacob, Turkekul, Mesruh, Barlas, Afsar, Yarilin, Dmitry, Fujisawa, Sho, Fan, Ning, Brendel, Matthew, Zamarin, Dmitriy, Wolchok, Jedd D., Manova-Todorova, Katia
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2017
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5751444/
https://ncbi.nlm.nih.gov/pubmed/28185198
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6759-9_18
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