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Efficient solubilization and purification of highly insoluble membrane proteins expressed as inclusion bodies using perfluorooctanoic acid

The purification of membrane proteins can be challenging due to their low solubility in conventional detergents and/or chaotropic solutions. The introduction of fusion systems that promote the formation of inclusion bodies has facilitated the overexpression of membrane proteins. In this protocol, we...

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Bibliografische gegevens
Gepubliceerd in:Protein Expr Purif
Hoofdauteurs: Plucinsky, Sarah M., Root, Kyle T., Glover, Kerney Jebrell
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2017
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5750070/
https://ncbi.nlm.nih.gov/pubmed/29066155
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2017.10.012
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