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Efficient solubilization and purification of highly insoluble membrane proteins expressed as inclusion bodies using perfluorooctanoic acid

The purification of membrane proteins can be challenging due to their low solubility in conventional detergents and/or chaotropic solutions. The introduction of fusion systems that promote the formation of inclusion bodies has facilitated the overexpression of membrane proteins. In this protocol, we...

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Detalhes bibliográficos
Publicado no:Protein Expr Purif
Main Authors: Plucinsky, Sarah M., Root, Kyle T., Glover, Kerney Jebrell
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5750070/
https://ncbi.nlm.nih.gov/pubmed/29066155
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2017.10.012
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