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Efficient solubilization and purification of highly insoluble membrane proteins expressed as inclusion bodies using perfluorooctanoic acid
The purification of membrane proteins can be challenging due to their low solubility in conventional detergents and/or chaotropic solutions. The introduction of fusion systems that promote the formation of inclusion bodies has facilitated the overexpression of membrane proteins. In this protocol, we...
Gardado en:
| Publicado en: | Protein Expr Purif |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2017
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5750070/ https://ncbi.nlm.nih.gov/pubmed/29066155 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2017.10.012 |
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