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Efficient solubilization and purification of highly insoluble membrane proteins expressed as inclusion bodies using perfluorooctanoic acid
The purification of membrane proteins can be challenging due to their low solubility in conventional detergents and/or chaotropic solutions. The introduction of fusion systems that promote the formation of inclusion bodies has facilitated the overexpression of membrane proteins. In this protocol, we...
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| Gepubliceerd in: | Protein Expr Purif |
|---|---|
| Hoofdauteurs: | , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
2017
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5750070/ https://ncbi.nlm.nih.gov/pubmed/29066155 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2017.10.012 |
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