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T-cell calcium dynamics visualized in a ratiometric tdTomato-GCaMP6f transgenic reporter mouse

Calcium is an essential cellular messenger that regulates numerous functions in living organisms. Here, we describe development and characterization of ‘Salsa6f’, a fusion of GCaMP6f and tdTomato optimized for cell tracking while monitoring cytosolic Ca(2+), and a transgenic Ca(2+) reporter mouse wi...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Dong, Tobias X, Othy, Shivashankar, Jairaman, Amit, Skupsky, Jonathan, Zavala, Angel, Parker, Ian, Dynes, Joseph L, Cahalan, Michael D
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5747524/
https://ncbi.nlm.nih.gov/pubmed/29239725
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.32417
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