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Analysis of Disulfide Bond Formation
In this unit, protocols are provided for detection of disulfide bond formation in cultures of intact cells and in an in-vitro-translation system containing isolated microsomes or semi-permeabilized cells. First, the newly synthesized protein of interest is biosynthetically labeled with radioactive a...
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Publicado no: | Curr Protoc Protein Sci |
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Main Authors: | , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2017
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5743216/ https://ncbi.nlm.nih.gov/pubmed/29091273 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpps.43 |
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