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Analysis of Disulfide Bond Formation

In this unit, protocols are provided for detection of disulfide bond formation in cultures of intact cells and in an in-vitro-translation system containing isolated microsomes or semi-permeabilized cells. First, the newly synthesized protein of interest is biosynthetically labeled with radioactive a...

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Bibliographic Details
Published in:Curr Protoc Protein Sci
Main Authors: Braakman, Ineke, Lamriben, Lydia, van Zadelhoff, Guus, Hebert, Daniel N.
Format: Artigo
Language:Inglês
Published: 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5743216/
https://ncbi.nlm.nih.gov/pubmed/29091273
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpps.43
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