Wird geladen...
Cell-free Generation of COPII-coated Procollagen I Carriers
The aim of this protocol is to generate COPII-coated procollagen I (PC1) carriers in a cell-free reaction. The COPII-coated PC1 carriers were reconstituted from donor membrane, cytosol, purified recombinant COPII proteins, and nucleotides. This protocol describes the preparation of donor membrane an...
Gespeichert in:
| Veröffentlicht in: | Bio Protoc |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Bio-Protocol
2017
|
| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5738930/ https://ncbi.nlm.nih.gov/pubmed/29276723 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2450 |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|