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Bacillus subtilis DNA polymerases, PolC and DnaE, are required for both leading and lagging strand synthesis in SPP1 origin-dependent DNA replication

Firmicutes have two distinct replicative DNA polymerases, the PolC leading strand polymerase, and PolC and DnaE synthesizing the lagging strand. We have reconstituted in vitro Bacillus subtilis bacteriophage SPP1 θ-type DNA replication, which initiates unidirectionally at oriL. With this system we s...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Seco, Elena M., Ayora, Silvia
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2017
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5737612/
https://ncbi.nlm.nih.gov/pubmed/28575448
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx493
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