Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe

Protein kinase A was detected by quantifying the amount of ATP used after a protein kinase reaction. The ATP assay was performed using the T4 DNA ligase and a molecular beacon (MB). In the presence of ATP, DNA ligase catalyzed the ligation of short DNA. The ligation product then hybridized to MB, re...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:Bioengineered
Main Authors: Ma, Changbei, Lv, Xiaoyuan, Wang, Kemin, Jin, Shunxin, Liu, Haisheng, Wu, Kefeng, Zeng, Weimin
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2017
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5736335/
https://ncbi.nlm.nih.gov/pubmed/28594266
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/21655979.2017.1338219
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados