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In vitro reconstitution of chaperone-mediated human RISC assembly
To silence target mRNAs, small RNAs and Argonaute (Ago) proteins need to be assembled into RNA-induced silencing complexes (RISCs). Although the assembly of Drosophila melanogaster RISC was recently reconstituted by Ago2, the Dicer-2/R2D2 heterodimer, and five chaperone proteins, the absence of a re...
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| Publicat a: | RNA |
|---|---|
| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Cold Spring Harbor Laboratory Press
2018
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5733571/ https://ncbi.nlm.nih.gov/pubmed/28971854 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.063891.117 |
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