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Engineering peptide ligase specificity by proteomic identification of ligation sites
Enzyme-catalyzed peptide ligation is a powerful tool for site-specific protein bioconjugation, but stringent enzyme–substrate specificity limits its utility. Here, we present an approach for comprehensive characterization of peptide ligase specificity for N termini using proteome-derived peptide lib...
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| Publicado no: | Nat Chem Biol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5726896/ https://ncbi.nlm.nih.gov/pubmed/29155430 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2521 |
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