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An improved labeling strategy enables automated detection of single-virus fusion and assessment of HIV-1 protease activity in single virions

Enveloped viruses transfer their genomes into host cells by fusing their membrane to that of the cell. To visualize single-virus fusion in living cells, researchers take advantage of the proteolytic maturation of HIV, type 1 (HIV-1), which can generate free fluorescent proteins within the viral part...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Sood, Chetan, Francis, Ashwanth C., Desai, Tanay M., Melikyan, Gregory B.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5724006/
https://ncbi.nlm.nih.gov/pubmed/29046351
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M117.818088
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