High-Resolution Northern Blot for a Reliable Analysis of MicroRNAs and Their Precursors

This protocol describes how to perform northern blot analyses to detect microRNAs and their precursors with single-nucleotide resolution, which is crucial for analyzing individual length variants and for evaluating relative quantities of unique microRNAs in cells. Northern blot analysis consists of...

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Detalles Bibliográficos
Publicado en:ScientificWorldJournal
Autores principales: Koscianska, Edyta, Starega-Roslan, Julia, Czubala, Katarzyna, Krzyzosiak, Wlodzimierz J.
Formato: Artigo
Lenguaje:Inglês
Publicado: TheScientificWorldJOURNAL 2011
Materias:
Peer-Reviewed Protocol
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5720006/
https://ncbi.nlm.nih.gov/pubmed/21218267
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1100/tsw.2011.11
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