Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2

Optogenetic tools allow regulation of cellular processes with light, which can be delivered with spatiotemporal resolution. In previous work, we used cryptochrome 2 (CRY2) and CIB1, Arabidopsis proteins that interact upon light illumination, to regulate transcription with light in yeast. While adopt...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Autores principales: Pathak, Gopal P., Spiltoir, Jessica I., Höglund, Camilla, Polstein, Lauren R., Heine-Koskinen, Sari, Gersbach, Charles A., Rossi, Jari, Tucker, Chandra L.
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2017
Materias:
Methods Online
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5714224/
https://ncbi.nlm.nih.gov/pubmed/28431041
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx260
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