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Directed Evolution of Recombinant C-Terminal Truncated Staphylococcus epidermidis Lipase AT2 for the Enhancement of Thermostability

In the industrial processes, lipases are expected to operate at temperatures above 45 °C and could retain activity in organic solvents. Hence, a C-terminal truncated lipase from Staphylococcus epidermis AT2 (rT-M386) was engineered by directed evolution. A mutant with glycine-to-cysteine substitutio...

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Detalhes bibliográficos
Publicado no:Int J Mol Sci
Main Authors: Veno, Jiivittha, Ahmad Kamarudin, Nor Hafizah, Mohamad Ali, Mohd Shukuri, Masomian, Malihe, Raja Abd. Rahman, Raja Noor Zaliha
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5713198/
https://ncbi.nlm.nih.gov/pubmed/29113034
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms18112202
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