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Cell-free Fluorescent Intra-Golgi Retrograde Vesicle Trafficking Assay

Intra-Golgi retrograde vesicle transport is used to traffic and sort resident Golgi enzymes to their appropriate cisternal locations. An assay was established to investigate the molecular details of vesicle targeting in a cell-free system. Stable cell lines were generated in which the trans-Golgi en...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Cottam, Nathanael P., Ungar, Daniel
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5706631/
https://ncbi.nlm.nih.gov/pubmed/29201946
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2616
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