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Optimization of PCR conditions for amplifying an AT-rich amino acid transporter promoter sequence with high number of tandem repeats from Arabidopsis thaliana

OBJECTIVE: The aim of the present study is to optimize the PCR conditions required to amplify the promoter sequence of an amino acid transporter having an AT-rich base composition with a high number of tandem repeats. RESULT: Results show that successful amplification can be achieved by performing a...

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Publicado en:BMC Res Notes
Autores principales: Dhatterwal, Pinky, Mehrotra, Sandhya, Mehrotra, Rajesh
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2017
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5706289/
https://ncbi.nlm.nih.gov/pubmed/29183338
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-017-2982-1
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