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Cell-free protein synthesis from non-growing, stressed Escherichia coli

Cell-free protein synthesis is a versatile protein production system. Performance of the protein synthesis depends on highly active cytoplasmic extracts. Extracts from E. coli are believed to work best; they are routinely obtained from exponential growing cells, aiming to capture the most active tra...

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Detalles Bibliográficos
Publicado en:Sci Rep
Main Authors: Failmezger, Jurek, Rauter, Michael, Nitschel, Robert, Kraml, Michael, Siemann-Herzberg, Martin
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5705671/
https://ncbi.nlm.nih.gov/pubmed/29184159
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-16767-7
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