Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing

Writing specific DNA sequences into the human genome is challenging with non-viral gene-editing reagents, since most of the edited sequences contain various imprecise insertions or deletions. We developed a modular RNA aptamer-streptavidin strategy, termed S1mplex, to complex CRISPR-Cas9 ribonucleop...

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Vydáno v:Nat Commun
Hlavní autoři: Carlson-Stevermer, Jared, Abdeen, Amr A., Kohlenberg, Lucille, Goedland, Madelyn, Molugu, Kaivalya, Lou, Meng, Saha, Krishanu
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2017
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5700129/
https://ncbi.nlm.nih.gov/pubmed/29167458
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-01875-9
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