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Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1
The bacteria-derived clustered regularly interspaced short palindromic repeat (CRISPR)–Cas systems are powerful tools for genome engineering. Recently, in addition to Cas protein engineering, the improvement of guide RNAs are also performed, contributing to broadening the research area of CRISPR–Cas...
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| Veröffentlicht in: | Nat Commun |
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| Hauptverfasser: | , , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Publishing Group UK
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5700056/ https://ncbi.nlm.nih.gov/pubmed/29167440 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-01650-w |
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