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Evaluation and optimization of reduction and alkylation methods to maximize peptide identification with MS-based proteomics

Mass spectrometry (MS) has become an increasingly important technique to analyze proteins. In popular bottom-up MS-based proteomics, reduction and alkylation are routine steps to facilitate peptide identification. However, the reaction incompletion and side reactions may occur, which compromise the...

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Detalhes bibliográficos
Publicado no:Mol Biosyst
Main Authors: Suttapitugsakul, Suttipong, Xiao, Haopeng, Smeekens, Johanna, Wu, Ronghu
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5698164/
https://ncbi.nlm.nih.gov/pubmed/29019370
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7mb00393e
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