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Evaluation and optimization of reduction and alkylation methods to maximize peptide identification with MS-based proteomics
Mass spectrometry (MS) has become an increasingly important technique to analyze proteins. In popular bottom-up MS-based proteomics, reduction and alkylation are routine steps to facilitate peptide identification. However, the reaction incompletion and side reactions may occur, which compromise the...
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Publicado no: | Mol Biosyst |
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Main Authors: | , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2017
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5698164/ https://ncbi.nlm.nih.gov/pubmed/29019370 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7mb00393e |
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