Mechanism of oxidative conversion of Amplex(®) Red to resorufin: pulse radiolysis and enzymatic studies

Amplex(®) Red (10-acetyl-3,7-dihydroxyphenoxazine) is a fluorogenic probe widely used to detect and quantify hydrogen peroxide in biological systems. Detection of hydrogen peroxide is based on peroxidase-catalyzed oxidation of Amplex(®) Red to resorufin. In this study we investigated the mechanism o...

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Publié dans:Free Radic Biol Med
Auteurs principaux: Dębski, Dawid, Smulik, Renata, Zielonka, Jacek, Michałowski, Bartosz, Jakubowska, Małgorzata, Dębowska, Karolina, Adamus, Jan, Marcinek, Andrzej, Kalyanaraman, Balaraman, Sikora, Adam
Format: Artigo
Langue:Inglês
Publié: 2016
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5697983/
https://ncbi.nlm.nih.gov/pubmed/27021961
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2016.03.027
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