Quantification and phenotypic characterisation of peripheral IFN-γ producing leucocytes in chickens vaccinated against Newcastle disease

The aim of this study was to optimise and evaluate an intracellular cytokine staining (ICS) assay for assessment of T cell IFN-γ responses in chickens vaccinated against Newcastle disease (ND). We aimed to validate currently available antibodies to chicken IFN-γ using transfected CHO cells. Moreover...

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Détails bibliographiques
Publié dans:Vet Immunol Immunopathol
Auteurs principaux: Andersen, S.H., Vervelde, L., Sutton, K., Norup, L.R., Wattrang, E., Juul-Madsen, H.R., Dalgaard, T.S.
Format: Artigo
Langue:Inglês
Publié: Elsevier Scientific 2017
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5697524/
https://ncbi.nlm.nih.gov/pubmed/29129224
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.vetimm.2017.10.001
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