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Trap(Seq): An RNA Sequencing-based pipeline for the identification of genetrap insertions in mammalian cells
The development of haploid mammalian cell lines, coupled to next generation sequencing, has recently facilitated forward genetic screenings in mammals. For mutagenesis, retrovirus- or transposon-based genetraps are frequently used. Current methods to map genetrap insertions are based on inverse- or...
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| Udgivet i: | J Mol Biol |
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| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5695663/ https://ncbi.nlm.nih.gov/pubmed/28782559 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2017.07.020 |
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