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Trap(Seq): An RNA Sequencing-based pipeline for the identification of genetrap insertions in mammalian cells

The development of haploid mammalian cell lines, coupled to next generation sequencing, has recently facilitated forward genetic screenings in mammals. For mutagenesis, retrovirus- or transposon-based genetraps are frequently used. Current methods to map genetrap insertions are based on inverse- or...

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Detalhes bibliográficos
Publicado no:J Mol Biol
Main Authors: Mayor-Ruiz, Cristina, Dominguez, Orlando, Fernandez-Capetillo, Oscar
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5695663/
https://ncbi.nlm.nih.gov/pubmed/28782559
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2017.07.020
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