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Impact of plasmid architecture on stability and yEGFP3 reporter gene expression in a set of isomeric multicopy vectors in yeast

Multicopy episomal plasmids in yeast, used whenever elevated levels of foreign or homologous gene expression are necessary, are known to be less stable compared to the endogenous 2-μm plasmid they are based on, at least without selective pressure. Considering that rich medium favors growth rate and,...

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Detalhes bibliográficos
Publicado no:Appl Microbiol Biotechnol
Main Authors: Hohnholz, Ruben, Pohlmann, Kim Julia, Achstetter, Tilman
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Berlin Heidelberg 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5694506/
https://ncbi.nlm.nih.gov/pubmed/29052760
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-017-8558-0
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