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Automated Solid-Phase Protein Modification with Integrated Enzymatic Digest for Reaction Validation: Application of a Compartmented Microfluidic Reactor for Rapid Optimization and Analysis of Protein Biotinylation

Protein modification by covalent coupling of small ligands or markers is an important prerequisite for the use of proteins in many applications. Well-known examples are the use of proteins with fluorescent markers in many in vivo experiments or the binding of biotinylated antibodies via biotin–strep...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Front Bioeng Biotechnol
Main Authors: Fraas, Regina, Diehm, Juliane, Franzreb, Matthias
Format: Artigo
Jezik:Inglês
Izdano: Frontiers Media S.A. 2017
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5693853/
https://ncbi.nlm.nih.gov/pubmed/29181376
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2017.00072
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