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cGAL, a Temperature-Robust GAL4-UAS System for C. elegans

The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in C. elegans has not been described. Here we systematically optimize the system’s three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C....

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Bibliografiske detaljer
Udgivet i:Nat Methods
Main Authors: Wang, Han, Liu, Jonathan, Gharib, Shahla, Chai, Cynthia M., Schwarz, Erich M., Pokala, Navin, Sternberg, Paul W.
Format: Artigo
Sprog:Inglês
Udgivet: 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5693259/
https://ncbi.nlm.nih.gov/pubmed/27992408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4109
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