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Generation of Expandable, Multipotent Induced Cardiac Progenitor Cells from Mouse Fibroblasts and Potency Testing in ex vivo Embryos
Here, we describe a protocol to generate expandable and multipotent induced cardiac progenitor cells (iCPCs) from mouse adult fibroblasts using forced expression of Mesp1, Tbx5, Gata4, Nkx2.5, Baf60c (MTGNB) along with activation of Wnt and JAK/STAT signalling. This method does not use iPS cell fact...
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| Publicado no: | Nat Protoc |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5693216/ https://ncbi.nlm.nih.gov/pubmed/28426026 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.021 |
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