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Redox-sensitive GFP fusions for monitoring the catalytic mechanism and inactivation of peroxiredoxins in living cells

Redox-sensitive green fluorescent protein 2 (roGFP2) is a valuable tool for redox measurements in living cells. Here, we demonstrate that roGFP2 can also be used to gain mechanistic insights into redox catalysis in vivo. In vitro enzyme properties such as the rate-limiting reduction of wild type and...

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Bibliografiske detaljer
Udgivet i:Redox Biol
Main Authors: Staudacher, Verena, Trujillo, Madia, Diederichs, Tim, Dick, Tobias P., Radi, Rafael, Morgan, Bruce, Deponte, Marcel
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5684490/
https://ncbi.nlm.nih.gov/pubmed/29128826
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.redox.2017.10.017
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