Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro

Three-dimensional aggregation and organ culture methods are critical for recreating in vivo cellular phenomena outside the body. Previously, we used the conventional gas liquid interphase organ culture method to induce complete mouse spermatogenesis. After incorporating microfluidic systems, we achi...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Komeya, Mitsuru, Hayashi, Kazuaki, Nakamura, Hiroko, Yamanaka, Hiroyuki, Sanjo, Hiroyuki, Kojima, Kazuaki, Sato, Takuya, Yao, Masahiro, Kimura, Hiroshi, Fujii, Teruo, Ogawa, Takehiko
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2017
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5684205/
https://ncbi.nlm.nih.gov/pubmed/29133858
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-15799-3
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