Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies

Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease. We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EV...

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Bibliografske podrobnosti
izdano v:Sci Rep
Main Authors: Benedikter, Birke J., Bouwman, Freek G., Vajen, Tanja, Heinzmann, Alexandra C. A., Grauls, Gert, Mariman, Edwin C., Wouters, Emiel F. M., Savelkoul, Paul H., Lopez-Iglesias, Carmen, Koenen, Rory R., Rohde, Gernot G. U., Stassen, Frank R. M.
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group UK 2017
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5681555/
https://ncbi.nlm.nih.gov/pubmed/29127410
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-15717-7
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